Nucleotide sequence of the region coding for 100K and 33K proteins of human enteric adenovirus type 41 (Tak).

Previous sequencing (1, 2) of human adenovirus type 41 strain Tak (3) DNA only partially identified the regions coding for L4 100K and 33K proteins. These two proteins are involved in vinon morphogenesis (4, 5). We have now completed this identification by cloning an Ad41 3.1 Kb Mstl-EcoRI fragment (map position 64.9% to 74%) in pBluescnptn and sequencing it directly using custom ohgonucleotide primers. By sequence analysis and GenBank homology searches we identified an open reading frame of 777 amino acids (base 125 to 2458) coding for the 100K protein. For the 33 K protein gene (bases 2145-2976), consensus donor and acceptor splice sequences were located, which predict the removal of a 178 nucleotides long intron from 33K protein gene transcripts (bases 2503 to 2680) as observed for Ad2 (5). In addition, five more ORFs were identified (the last three on the complementary strand): (i) 16.5K (bases 480-911) (ii) 7.3K